Semi- Prep Preparative Columns

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Product Details:

Automation Grade Manual operation
Mounting Type Direct mounting in HPLC system
Port Size 1/16 inch standard fitting
Response Time Immediate chromatographic response dependent on system
Equipment Type Preparative HPLC Column
Features High theoretical plate number, excellent mechanical strength, reusable design, compatibility with wide range of solvents
Operating Voltage Not required
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Semi- Prep Preparative Columns Price And Quantity

Minimum Order Quantity 3-4 Piece

Semi- Prep Preparative Columns Product Specifications

Hardness Column housing: Stainless Steel or PEEK
Specimen Size Depends on column ID (typically up to 1 g sample)
Measuring Range Varies by column dimensions (typically 100 to 300 pore size)
Power Supply Not Required
Control Mode Manual
Resolution Optimized for high resolution purification
Accuracy High chemical and mechanical stability for reproducible results
Interface Type 1/16 standard ferrule connection
Operating Voltage Not required
Number of Specimens Single per column
Features High theoretical plate number, excellent mechanical strength, reusable design, compatibility with wide range of solvents
Capacity 5 mL, 10 mL, 22 mL, or as specified
Response Time Immediate chromatographic response dependent on system
Equipment Type Preparative HPLC Column
Usage Laboratory HPLC instrument
Mounting Type Direct mounting in HPLC system
Automation Grade Manual operation
Port Size 1/16 inch standard fitting

Semi- Prep Preparative Columns Trade Information

Supply Ability 3-4 Piece Per Day
Delivery Time 3-4 Week

Product Description

Semi-Prep Preparative Columns are columns that are used to purify and isolate compounds from samples that contain a mixture of compounds. These columns are made from high-quality silica gel, with a variety of bonded phase types available, and are designed for the efficient separation of up to a few grams of sample. The columns are packed with a high column capacity, providing superior performance and higher resolution. Semi-Prep Preparative Columns are ideal for preparative chromatography and purification of compounds in a variety of industries, including pharmaceutical, biotechnology, food, and environmental analysis.

Versatile Design for Enhanced Laboratory Use

Choose from stainless steel or PEEK constructions with varying internal diameters and lengths to suit your specific HPLC purification needs. With robust end fittings and column housings, these columns maintain integrity even under high pressure and demanding lab conditions, delivering accuracy and reproducibility for analytical tasks.

Efficient Purification with Reliable Operation

Designed for manual control with direct mounting capability, the columns feature optimized flow rates up to 100 mL/min. The reusable nature and compatibility with a broad spectrum of solvents, including acetonitrile, methanol, and water, make these columns suitable for both routine and specialized laboratory procedures.

FAQs of Semi- Prep Preparative Columns:

Q: How do I select the appropriate column size for my application?

A: Column size selection depends on your sample volume, required resolution, and intended throughput. Internal diameters range from 5 mm to 30 mm, and lengths from 50 mm to 250 mm or custom. Larger IDs and lengths accommodate higher sample capacities and flow rates, making them ideal for preparative purification.

Q: What is the recommended process for installing these columns into an HPLC system?

A: The columns are designed for direct mounting via 1/16 inch standard ferrule connections. Ensure your HPLC system supports these fittings, connect the column with care using PTFE or Vespel ferrules for leak-free sealing, and verify the flow rate matches the columns specifications before starting chromatographic runs.

Q: When should I consider stainless steel versus PEEK for the column material?

A: Stainless steel is recommended for applications requiring maximum mechanical strength and compatibility with aggressive solvents. PEEK is preferred in cases where metal-sensitive samples are used or when working with milder solvents and lower pressure conditions.

Q: Where can these semi-prep columns be used?

A: These columns are suitable for laboratory HPLC instruments in research, pharmaceutical, academic, or quality control environments, supporting processes that demand high-resolution purification and consistent performance.

Q: What are the benefits of using a column supplied with an individual test chromatogram?

A: Each column comes with a test chromatogram to certify performance, helping you verify purity, resolution, and reproducibility. This ensures reliable results and confidence in the integrity of your purification application.

Q: How is solvent compatibility ensured with these preparative columns?

A: Engineered with materials resistant to common solvents such as acetonitrile, methanol, and water, along with durable end fittings and seals, these columns support a wide range of mobile phases for diverse preparative tasks.

Q: What maintenance is required for reusable semi-prep columns?

A: Routine rinsing with compatible solvents, periodic inspection of end fittings and ferrules, and adherence to recommended pressure and flow limits will help maintain column performance and extend operational lifespan.

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