Product Description
Thesemini spin columns are useful for samples, including E Coli, agarose gel/PCRproducts, serum plasma, tissue, and whole blood.
With theright filter, you can extract various DNAs, including Plasmid DNA, DNAfragments, Viral DNA/RNA, and Genomic DNA
Efficient Purification and FiltrationDesigned for high throughput, our 50mL spin column harnesses the power of centrifugal force to rapidly filter and purify nucleic acids and proteins. The silica membrane delivers over 90% nucleic acid recovery, enabling highly accurate extraction from a variety of sample types, including blood and tissue lysates. The disposable format also reduces contamination risks.
Reliable Design for Laboratory UseBuilt from robust polypropylene, each spin column provides secure, leak-proof operation. The snap cap is conveniently attached, offering effortless sealing and minimizing the chance of spills during centrifugation. Options for either a 0.22m or 0.45m membrane and colored caps are available, supporting diverse protocols and sample tracking.
FAQs of Spin column for 50mL tube:
Q: How is the 50mL spin column typically used in the laboratory?
A: The 50mL spin column is mainly used for DNA, RNA, and protein purification, as well as for filtration and extraction applications. Samples such as blood, cells, or lysates are loaded into the column, and then centrifugation is applied to achieve rapid purification through the silica membrane.
Q: What types of samples are compatible with this spin column?
A: This spin column is suitable for a wide range of samples, including blood, cultured cells, tissue lysate, and various environmental samples. Its design accommodates up to 50 mL of sample, making it versatile for different research needs.
Q: When should I choose between the 0.22m and 0.45m membrane pore sizes?
A: Choose a 0.22m membrane for finer filtration, often required in sensitive DNA or protein purification to remove smaller particulates or microorganisms. The 0.45m option is suitable for less stringent applications where higher flow rates are desired.
Q: Where can I source pre-sterilized spin columns?
A: While standard columns are supplied non-sterile, pre-sterilized versions can be requested through your distributor, supplier, or manufacturer when placing your order for specific sterile workflow requirements.
Q: How does the snap cap design benefit my workflow?
A: The attached snap cap provides a secure and leak-proof seal, reducing the risk of sample contamination or spillage during transport, centrifugation, and storage. This streamlined design supports safe and convenient handling.
Q: Is this spin column reusable?
A: No, the 50mL spin column is strictly intended for single use. This ensures optimal purity of results and minimizes the possibility of cross-sample contamination, which is crucial in sensitive molecular biology protocols.
Q: What are the main advantages of using this spin column for nucleic acid extractions?
A: The spin column offers high recovery rates (over 90%), rapid filtration, leak-proof reliability, and compatibility with various sample types. Its disposable format and clear construction further enhance workflow efficiency and result integrity.