Leibovitz's L-15 Medium, no phenol red

5000 INR

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Product Details:

Automation Grade Manual
Capacity 500 mL, 1 L, 10 L (varies by packaging)
Feature Prepared without phenol red to avoid estrogenic effects; suitable for sensitive assays
Temperature Range Store at 2C to 8C
Core Components Amino acids, vitamins, inorganic salts, carbohydrates, L-glutamine, no phenol red
Model No As referenced by manufacturer (varies by supplier)
Equipment Materials Sterile filtered solution in PET or glass bottles
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Leibovitz's L-15 Medium, no phenol red Price And Quantity

Minimum Order Quantity 1 Piece
Price 5000 INR

Leibovitz's L-15 Medium, no phenol red Product Specifications

Model No As referenced by manufacturer (varies by supplier)
Dimension (L*W*H) Standard bottle dimensions, varies by volume
Temperature Range Store at 2C to 8C
Core Components Amino acids, vitamins, inorganic salts, carbohydrates, L-glutamine, no phenol red
Automation Grade Manual
Equipment Materials Sterile filtered solution in PET or glass bottles
Feature Prepared without phenol red to avoid estrogenic effects; suitable for sensitive assays
Usage For in vitro cultivation of cells and tissue culture
Type Cell Culture Medium
Weight Varies by packaging (e.g., ~600g for 500 mL bottle)
Capacity 500 mL, 1 L, 10 L (varies by packaging)

Leibovitz's L-15 Medium, no phenol red Trade Information

Supply Ability 5-10 Piece piece Per Month
Delivery Time 6-8 Week

Product Description

Leibovitz's L-15 Medium, no phenol red

Description

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO₂ equilibration.

This L-15 is modified as follows:

With	Without
Galactose	Glucose
L-glutamine	Phenol Red
Sodium Pyruvate	HEPES
	Sodium Bicarbonate

The complete formulation is available.

Using L-15
L-15 contains no proteins, lipids, or growth factors. Therefore, L-15 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

cGMP manufacturing and quality system
Leibovitz's L-15 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Ready-to-Use, Phenol Red-Free Medium

Leibovitzs L-15 Medium is formulated without phenol red, making it particularly well-suited for assays where estrogenic interference is a concern. The liquid, ready-to-use nature streamlines workflows in tissue culture laboratories, especially when preparing sensitive cell lines or conducting virus and vaccine research.

Ensured Quality and Safety

Manufactured sterile and mycoplasma tested, this medium boasts a low endotoxin level of 1 EU/mL, supporting reliable results in rigorous experimental setups. Each batch is produced under stringent quality controls and comes with a 12-month shelf life when stored as recommended, ensuring both safety and consistency.

Flexible Packaging for Diverse Applications

Available in 500 mL, 1 L, and 10 L formats, Leibovitzs L-15 Medium meets various scale requirements. The packaging in PET or glass bottles maintains the sterility of the product, making it adaptable for different lab settings and storage needs. Its standard bottle dimensions allow for easy integration into existing workflows.

FAQs of Leibovitzs L-15 Medium, no phenol red:

Q: How should Leibovitzs L-15 Medium, no phenol red, be stored to maintain its quality?

A: The medium should be stored at temperatures between 2C and 8C, shielded from light. When left unopened and properly stored, it retains its quality for up to 12 months from the date of manufacture.

Q: What types of applications are suitable for this medium?

A: Leibovitzs L-15 Medium is appropriate for mammalian cell culture, virus and vaccine research, and explant culture. Its phenol red-free formulation also makes it ideal for sensitive assays where minimizing estrogenic activity is critical.

Q: When is it beneficial to use a cell culture medium without phenol red?

A: Using a medium prepared without phenol red is advantageous in experiments where estrogenic effects could interfere with results, such as hormone sensitivity assays or studies focusing on cellular responses to hormones.

Q: Where does the medium typically fit into the laboratory workflow?

A: Leibovitzs L-15 Medium is used during the in vitro cultivation of cells and tissues, serving as a nutrient-rich environment for growth, maintenance, and assay procedures in both research and production laboratories.

Q: What is the process for using this medium with cell cultures?

A: Simply aseptically transfer the required volume of the ready-to-use medium into the culture vessel containing cells or tissue samples. No additional preparation or supplementation is necessary unless specified by your protocol.

Q: What are the core components of Leibovitzs L-15 Medium, and why are they important?

A: The medium contains essential amino acids, vitamins, inorganic salts, carbohydrates, and L-glutamine, all vital for supporting robust cell growth, metabolic activity, and experimental consistency.

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