Product Description
HPLC Columns
State-of-the-art Reversed-Phase and HILIC HPLC Columns
Waters offers a wide range of analytical and preparative chromatography columns to meet the needs of virtually every application. The Waters Column Advisor recommends the most appropriate column depending on the on the application requirements you need to consider and the column characteristics you are looking for. Separations developed on our analytical columns can be directly scaled up to preparative columns. We supply a wide range of phase chemistries in numerous column dimensions for preparative work to meet your scale-up needs. All chromatography columns meet stringent specifications to ensure excellent performance and column-to-column reproducibility. We also offer a wide range of accessories to support these columns.
Optimized for Reliable SeparationEquipped with C18 packing and a 5 m particle size, this column ensures precise separation of complex mixtures. Suitable for use in pharmaceuticals and chemical analysis, its high efficiency and mechanical strength contribute to consistent, reliable outcomes for demanding HPLC tasks.
Robust Design for Versatile ApplicationsManufactured using stainless steel and silica-based materials, the WATERS HPLC COLUMN offers exceptional robustness. It is engineered to withstand pressures up to 400 bar and temperatures up to 60C, ensuring durability and stability throughout rigorous analytical procedures.
Universal Compatibility and Ease of UseFitted with standard 1/16-inch end fittings, the column integrates smoothly with most HPLC and UPLC systems across laboratories. Its USP Type L1 classification and cylindrical shape enhance its suitability for a broad spectrum of analytical methods.
FAQs of WATERS HPLC COLUMN:
Q: How is the WATERS HPLC COLUMN typically used in laboratory applications?
A: This column is primarily employed for the separation and analysis of compounds in pharmaceuticals, chemicals, and biological samples using high-performance liquid chromatography techniques.
Q: What are the main benefits of choosing a column with C18 reversed-phase packing material?
A: C18 (Octadecyl) packing provides high efficiency and selectivity for a wide range of analytes, making it ideal for routine and complex separations and ensuring reproducible performance across different sample types.
Q: When should I use this column instead of other USP L1 columns?
A: Opt for this column when high analytical accuracy, robust design, and compatibility with most HPLC/UPLC systems are crucial, particularly for applications requiring separation of diverse compounds under reversed-phase conditions.
Q: Where can this column be installed and used effectively?
A: The column can be installed in any compatible HPLC or UPLC instrument equipped with standard 1/16-inch end fittings. It is widely used in research labs, pharmaceutical companies, chemical analysis facilities, and academic institutions.
Q: What process is involved in the preparation and installation of the column for analysis?
A: Typically, you should flush the column with appropriate solvents to equilibrate it, connect to the HPLC system using standard fittings, and follow manufacturer guidelines for optimal performance prior to analytical runs.
Q: How does the column design contribute to high analytical accuracy?
A: The precision of the 5 m particle size, 100 pore size, and silica-based C18 packing ensures uniform flow and consistent separation efficiency, contributing to accurate and reproducible analytical results.
Q: What are the temperature and pressure limits for operating this HPLC column?
A: The column tolerates pressures up to approximately 400 bar and temperatures up to 60C, accommodating various HPLC operating conditions and solvent systems while maintaining structural integrity.