
Strip
Strip is a conventional spore strip biological indicator for monitoring steam, low-temperature steam formaldehyde, ethylene oxide, and radiation industrial sterilization processes. Strip contains bacterial spores on a paper carrier sealed within a convenient, peel-open glassine envelope. To use a Strip, place the glassine envelope in a product or product package, then place the inoculated package in a sterilizer and process normally. Upon removal from the sterilizer, send the inoculated package to the microbiology laboratory where the Strip* is to be aseptically transferred to soybean casein digest culture medium and incubated at the appropriate temperature for 7 days.
Process | Spore | Incubation |
Steam | G. stearothermophilus | 7 days at 55-60C |
Gas | B. atrophaeus | 7 days at 30-35C |
Radiation | B. pumilus | 7 days at 30-35C |
LTS Formaldehyde | G. stearothermophilus | 7 days at 55-60C |
Dual Species | B. atrophaeus/G. stearothermophilus | |
Gas | 7 days at 30-35C | |
Steam | 7 days at 55-60C |
*The filter paper carrier must be aseptically removed from the glassine envelope in a Class 100 hood or biosafety cabinet. Strict aseptic practices and techniques must be adhered to when making the transfer to culture the biological indicator or false positive results may occur.
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