Product Description
Waters Spherisorb 5 m Columns and Cartridges
Columns Cartridge Columns
Chemistry 4.0 mm 4.0 mm 4.6 mm 4.6 mm 4.6 mm x4.6 mm 4.6 mm 4.6 mm 4.6 mm 4.6 mm
x 125 mm x 250 mm x 50 mm x 100 mm 150 mm x 250 mm x 50 mm x 100 mm x 150 mm x 250 mm
S5 ODS2 PSS845543 PSS845277 PSS831911 PSS831912 PSS831913 PSS831915 PSS839536 PSS839537 PSS839538 PSS839540
S5 ODS1 PSS845541 PSS845542 PSS830611 PSS830612 PSS830613 PSS830615 PSS839506 PSS839507 PSS839508 PSS839510
S5 ODS B PSS839613 PSS839615
S5 C8 PSS845280 PSS845281 PSS831811 PSS831812 PSS831813 PSS831815 PSS839531 PSS839532 PSS839533 PSS839535
S5 C6 PSS845284 PSS845285 PSS831011 PSS831012 PSS831013 PSS831015 PSS839521 PSS839522 PSS839523 PSS839525
S5 C1 PSS845288 PSS845289 PSS832611 PSS832612 PSS832613 PSS832615 PSS839566 PSS839567 PSS839568 PSS839570
S5 NH2 PSS845300 PSS845301 PSS831111 PSS831112 PSS831113 PSS831115 PSS839526 PSS839527 PSS839528 PSS839530
S5 P (Phenyl) PSS845292 PSS845293 PSS830812 PSS830813 PSS830815 PSS839511 PSS839512 PSS839513 PSS839515
S5 CN Normal PhaPSS845296 PSS845297 PSS830911 PSS830912 PSS830913 PSS830915 PSS839516 PSS839517 PSS839518 PSS839520
S5 CN Reversed Phase PSS830908 PSS830909
S5 W (Silica) PSS845539 PSS845540 PSS830111 PSS830112 PSS830113 PSS830115 PSS839502 PSS839503 PSS839505
Ion Exchange
S5 SAX PSS845304 PSS845305 PSS832711 PSS832712 PSS832713 PSS832715 PSS839571 PSS839572 PSS839573 PSS839575
S5 SCX PSS845308 PSS845309 PSS837511 PSS837512 PSS837513 PSS837515 PSS839651 PSS839652 PSS839653 PSS839655
Mixed Mode
S5 OD/CN PSS837812 PSS837813 PSS837815 PSS839643 PSS839645
Precision and Flexibility in HPLC ApplicationsWaters Spherisorb columns and cartridges provide consistent particle size and high purity silica for reliable separations. Their versatility is enhanced by a choice of stationary phases, including C18, C8, CN, silica, phenyl, SAX, and SCX, making them suitable for various analytical and preparative techniques. Endcapping and customizable dimensions further support method development and adaptation to specific research or industry requirements.
Robust Design for Demanding Laboratory EnvironmentsCrafted using stainless steel or glass, these columns ensure durability, chemical resistance, and compatibility with most HPLC equipment. Their cylindrical shape and standardized dimensions facilitate easy installation and replacement. Rated for high pressure and temperature resistance up to 60C, they maintain structural integrity for repeatable, accurate chromatographic performance.
Reproducible Performance and Broad Application RangeThese HPLC columns deliver high efficiency and reliable accuracy, thanks to their tightly controlled particle size and surface area. Ideal for qualitative and quantitative analyses, they excel in pharmaceutical, biochemical, environmental, and food testing laboratories. Their carbon loading, surface modification, and wide pH tolerance amplify separation selectivity and compatibility for many sample types.
FAQs of Waters Spherisorb 5 m Columns and Cartridges:
Q: How do I select the appropriate Waters Spherisorb column phase for my HPLC application?
A: Select the phase based on your analyte characteristics and separation goals. For nonpolar compounds, the C18 phase with 11% carbon load is suitable, while polar phases like CN and silica address different separation mechanisms. Phases such as phenyl, SAX, and SCX are available to enhance selectivity for aromatic or ionic species. Consult application guides or reach out to technical support for phase recommendations.
Q: What is the typical process for installing and using Waters Spherisorb 5 m columns in HPLC systems?
A: Installation involves securely connecting the column or cartridge (stainless steel or glass) to your HPLC instrument using compatible fittings. Pre-condition the column with mobile phase as recommended. During operation, maintain pressure below the maximum of 6000 psi (400 bar) and temperature below 60C for optimal efficiency and longevity.
Q: What are the benefits of choosing a 5 m particle size for chromatographic separations?
A: A 5 m particle size offers a balance between efficiency and pressure requirements, delivering high resolution without excessive backpressure. This supports reproducible performance and broad applicability in both analytical and preparative HPLC workflows.
Q: Where can I source Waters Spherisorb 5 m columns and cartridges in India?
A: You can obtain these columns and cartridges directly from authorized Waters distributors, exporters, importers, and manufacturers in India. Verified suppliers and traders are typically listed through leading laboratory equipment platforms and the Waters corporate website.
Q: When should different pore sizes (80 , 100 , 120 ) be used with these columns?
A: Select pore size based on the molecular size of your analytes. Use 80 for small molecules, while 100 and 120 are recommended for peptides, proteins, or larger biomolecules, ensuring optimal accessibility to the stationary phase and better separation.
Q: How does endcapping affect the performance of Waters Spherisorb columns?
A: Endcapping minimizes residual silanol interactions on the silica surface, reducing peak tailing for basic and polar analytes. This improves chromatographic efficiency, especially in reversed-phase applications, and the degree of endcapping varies by phase.
Q: What advantages do these columns offer in terms of reproducibility and compatibility?
A: Waters Spherisorb columns excel in reproducible separation results due to stringent manufacturing standards and surface area control. They are compatible with most HPLC systems and provide a wide application range across many industries, making them a dependable choice for routine and specialized analyses.