RPMI 1640 Medium, no phenol red

5000 INR

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Product Details:

Model No RPMI 1640, No Phenol Red
Core Components Amino acids, Vitamins, Inorganic salts, Glucose, Sodium bicarbonate, L-glutamine (optional), No phenol red
Temperature Range 2C to 8C (storage condition)
Feature No phenol red, ready-to-use, sterile-filtered, supports a wide range of mammalian cell lines
Capacity 500 ml, 1 L (custom volumes available)
Equipment Materials Sterile, tissue-culture grade plastic bottle
Type Cell Culture Medium
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RPMI 1640 Medium, no phenol red Price And Quantity

Price 5000 INR
Minimum Order Quantity 1 Piece

RPMI 1640 Medium, no phenol red Product Specifications

Type Cell Culture Medium
Equipment Materials Sterile, tissue-culture grade plastic bottle
Model No RPMI 1640, No Phenol Red
Feature No phenol red, ready-to-use, sterile-filtered, supports a wide range of mammalian cell lines
Core Components Amino acids, Vitamins, Inorganic salts, Glucose, Sodium bicarbonate, L-glutamine (optional), No phenol red
Dimension (L*W*H) Approx. 7cm x 7cm x 16cm (for 500 mL bottle)
Temperature Range 2C to 8C (storage condition)
Weight Approximately 550g (500 mL bottle)
Usage In vitro growth and maintenance of cells
Capacity 500 ml, 1 L (custom volumes available)

RPMI 1640 Medium, no phenol red Trade Information

Supply Ability 5-10 Piece piece Per Month
Delivery Time 6-8 Week

Product Description

RPMI 1640 Medium, no phenol red

Description

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI 1640 is modified as follows:

With	Without
L-glutamine	Phenol Red
	HEPES

The complete formulation is available.

Gibco RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B₁₂, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco RPMI 1640 product made in our Grand Island facility (11835-030). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

Glutamine:	L-Glutamine
Phenol Red Indicator:	No Phenol Red
Form:	Liquid
HEPES Buffer:	No HEPES
Serum Supplementation:	Standard Serum Supplementation
Sodium Pyruvate Additive:	No Sodium Pyruvate
Classification:	Animal Origin-Free
Concentrated:	1 X
Culture Environment:	CO2
Osmolality:	265 - 300 mOsm/kg
Product Line:	Gibco
Product Size:	500 mL
Sodium Bicarbonate Buffer:	Sodium Bicarbonate
Supplementation Required:	Serum
Volume (Metric):	500 ml
pH Range:	7.0 - 7.4
Green Features:	Fewer resources used, Less waste
Shipping Condition:	Room Temperature

Optimized for Sensitive Cell Cultures

Designed without phenol red, this RPMI 1640 variant is ideal for experiments where background color must be minimized, such as fluorescence-based assays. The clear medium maintains essential nutrients and physicochemical stability, supporting healthy cell growth across hybridoma, lymphocyte, and other mammalian cultures.

Flexible Supplementation and Applications

RPMI 1640 Medium can be readily adapted for various research needs. It accepts supplementation with 10% FBS, antibiotics, and L-glutamine, making it suitable for primary culture, maintenance, and cell-based assays. Its reliable performance has made it a trusted choice in laboratories globally.

FAQs of RPMI 1640 Medium, no phenol red:

Q: How should RPMI 1640 Medium, no phenol red be stored to maintain stability and sterility?

A: Store unopened bottles at 2C to 8C to preserve sterility, shelf life, and medium efficacy. Avoid repeated freeze-thaw cycles and always use aseptic techniques during handling.

Q: What kinds of cells and experiments is this medium most suitable for?

A: This medium is ideal for hybridoma, lymphocyte, and a variety of mammalian cell cultures, particularly in applications sensitive to indicator dyeslike fluorescence or absorbance-based assays.

Q: When do I need to supplement the medium, and with what components?

A: Supplement the medium as per your application requirements. Common additions include 10% fetal bovine serum (FBS), antibiotics to prevent contamination, and L-glutamine for enhanced cell metabolism.

Q: Where is RPMI 1640 Medium typically used in the research process?

A: It is primarily used in in vitro cell culture rooms or laboratories equipped with sterile hoods and incubators, supporting the growth and maintenance of cells for biological analysis.

Q: What is the process for using this medium in cell culture?

A: After equilibrating the medium to room temperature, aseptically supplement it if required. Add cells to an appropriate vessel, then introduce the medium, ensuring a sterile environment to avoid contamination.

Q: What are the benefits of using RPMI 1640 Medium without phenol red?

A: Excluding phenol red eliminates potential interference in assays sensitive to color changes, providing clearer results in fluorescence or colorimetric studies while maintaining optimum conditions for cell growth.

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Leibovitz's L-15 Medium, no phenol red Price : 5000 INR Minimum Order Quantity : 1 Equipment Materials : Sterile filtered solution in PET or glass bottles Type : Cell Culture Medium Feature : Prepared without phenol red to avoid estrogenic effects; suitable for sensitive assays Capacity : 500 mL, 1 L, 10 L (varies by packaging) Usage : For in vitro cultivation of cells and tissue culture Model No : As referenced by manufacturer (varies by supplier) Core Components : Amino acids, vitamins, inorganic salts, carbohydrates, Lglutamine, no phenol red Weight : Varies by packaging (e.g., ~600g for 500 mL bottle) Dimension (L*W*H) : Standard bottle dimensions, varies by volume Automation Grade : Manual Temperature Range : Store at 2C to 8C

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